In order to maintain a good linear relationship between the fluorescence intensity of the solution and the concentration of the solution, you should:
A. Keep the absorbance of the solution less than 0.05
B. Keep the light transmittance of the solution greater than 0.5
C. Use monochromatic light as excitation light
D. Use cuvettes with longer optical path
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To prepare 1000 mL 0.1 mol/L HCl solution, the volume of concentrated hydrochloric acid (12 mol/L) should be ( ).
A. 0.84 mL
B. 8.4 mL
C. 1.2 mL
D. 12 mL
Which of the following processes does not affect the non-radiative relaxation process of excited-state molecules?
A. Vibrational relaxation
B. Internal conversion
C. Quench
D. Aggregation
Which of the following factors generally does not affect the fluorescence spectrum of a solution?
A. Excitation wavelength
B. pH
C. Solvent
D. Concentration
Emission spectra are typically independent of the excitation wavelength. This is known as Kasha's rule.