题目内容

Which of the following factors generally does not affect the fluorescence spectrum of a solution?

A. Excitation wavelength
B. pH
C. Solvent
D. Concentration

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Emission spectra are typically independent of the excitation wavelength. This is known as Kasha's rule.

The fluorescence quantum yield is the ratio of the number of photons absorbed to the number of photons emitted.

In titration analysis, the sudden change of indicator color is generally used to determine the arrival of stoichiometric point, and the titration is stopped when the indicator changes color. This point is called ( ).

A. titration analysis
B. titration
C. stoichiometric point
D. endpoint of titration

Emission spectra vary widely and are dependent upon the chemical structure of the fluorophore and the solvent in which it is dissolved.

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