题目内容

What is the direction of genetic information flow of reverse transcription:____

A. DNA→DNA
B. DNA→RNA
C. RNA→DNA
DNA→protein
E. RNA→RNA

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What are the basic components of a nucleic acid:____

A. phosphate and ribose
B. nucleoside and nitrogenous base
C. mononucleotide
D. nitrogenous base
E. deoxynucleoside and base

Which of the following may cause non-specific amplification of DNA in PCR reaction?()

A. excessive TaqDNA polymerase
B. excessive primer
C. excessive Mg2+in buffer
D. either of A, B or C

Which of the following enzymes can tolerant high temperature:()

A. Taq DNA polymerase
B. HindIII
C. T4 ligase
D. RNA enzyme
E. Klenow segment

An archaeologist found the frozen muscle of an extinct giant animal in the ice of Siberian taiga region. He wanted to know the similarity of DNA of the giant animal and that of modern Indian elephant, and following detection work was done. The right steps and order is:①Lower the temperature and detect hybrid DNA region with double strand ② amplify DNA from giant animal and elephant by PCR ③ transducer DNA from giant animal into elephant cells ④ DNA from giant animal and elephant are heated together in the water bath under a certain temperature()

A. ②④③
B. ②④③①
C. ③④①
D. ②④①