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An archaeologist found the frozen muscle of an extinct giant animal in the ice of Siberian taiga region. He wanted to know the similarity of DNA of the giant animal and that of modern Indian elephant, and following detection work was done. The right steps and order is:①Lower the temperature and detect hybrid DNA region with double strand ② amplify DNA from giant animal and elephant by PCR ③ transducer DNA from giant animal into elephant cells ④ DNA from giant animal and elephant are heated together in the water bath under a certain temperature()

A. ②④③
B. ②④③①
C. ③④①
D. ②④①

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The denaturation temperature of PCR is generally:()

A. 95℃
B. 85℃
C. 72℃
D. 55℃
E. 100℃

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In genetic engineering, the target genes (1000bp, including 460 adenine deoxynucleotides) are amplified for 4 generation in the PCR instrument, the number of cytosine deoxynucleotides in PCR buffer should be at least:()

A. 640
B. 8100
C. 600
D. 8640

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The wrong statement about PCR is:()

A. PCR contains three steps including denaturation, annealing and extension
B. PCR technique can be used for gene diagnosis, determination of the genetic relationship etc.
C. The DNA polymerase used in PCR can be stored at room temperature due to its tolerance of higher temperature.
D. Sanger method for DNA sequencing is based on PCR technique.

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Polymerase chain reaction namely PCR is a technique that amplifies DNA fragments rapidly in vitro. Generally, the process of PCR contains the following 30 times cycles: denaturation of template DNA at 95℃ → annealing (primer binds to DNA template) at 55℃ → extension to form the nascent DNA chain at 72℃. Which of the following statement is NOT true about PCR process:()

A. In PCR process, both of the two strands of DNA are synthesized continuously, the same as synthesis of leading strand in DNA replication.
B. The binding of primer with DNA template strand depends on the principle of complementary base pairing in melting process.
C. The process of extension requires DNA polymerase, ATP and four kinds of ribonucleotides
D. The optimal temperature of DNA polymerase in PCR process is higher compared with DNA replication in cells.

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A. ②④③ B. ②④③① C. ③④① D. ②④①

The denaturation temperature of PCR is generally:()

In genetic engineering, the target genes (1000bp, including 460 adenine deoxynucleotides) are amplified for 4 generation in the PCR instrument, the number of cytosine deoxynucleotides in PCR buffer should be at least:()

The wrong statement about PCR is:()

A. ②④③
B. ②④③①
C. ③④①
D. ②④①