题目内容

The wrong statement about PCR is:()

A. PCR contains three steps including denaturation, annealing and extension
B. PCR technique can be used for gene diagnosis, determination of the genetic relationship etc.
C. The DNA polymerase used in PCR can be stored at room temperature due to its tolerance of higher temperature.
D. Sanger method for DNA sequencing is based on PCR technique.

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Polymerase chain reaction namely PCR is a technique that amplifies DNA fragments rapidly in vitro. Generally, the process of PCR contains the following 30 times cycles: denaturation of template DNA at 95℃ → annealing (primer binds to DNA template) at 55℃ → extension to form the nascent DNA chain at 72℃. Which of the following statement is NOT true about PCR process:()

A. In PCR process, both of the two strands of DNA are synthesized continuously, the same as synthesis of leading strand in DNA replication.
B. The binding of primer with DNA template strand depends on the principle of complementary base pairing in melting process.
C. The process of extension requires DNA polymerase, ATP and four kinds of ribonucleotides
D. The optimal temperature of DNA polymerase in PCR process is higher compared with DNA replication in cells.

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PCR is an enzymatic reaction that depends on DNA polymerase, with the presence of primer, template and four kinds of deoxyribonucleotides. The key factor to determine its specificity is:()

A. template
B. primer
C. dNTP
D. Mg2+

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The necessary conditions for PCR to amplify DNA is ①target gene ②primer③four deoxynucleotide ④DNA polymerase ⑤mRNA⑥ribosome()

A. ①②③④
B. ②③④⑤
C. ①③④⑤
D. ①②③⑥

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After sheep Dolly was born, it is doubtable that Dolly was not from somatic cell of an adult sheep, but from polluted embryonic cell in the experiment. The evidence that supports Dolly is from somatic cell rather than embryonic cell is:()

A. DNA fingerprint of Dolly
B. DNA melting temperature of Dolly
C. Telomere length of Dolly
D. Biological clock of Dolly

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