The HGP (Human Genome Project) was finished in:
A. 1990
B. 2001
C. 2008
D. 2006
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Several RNAs, which are very important for the development of the Drosophila embryo, are distributed in different regions of the embryo.Use () to prove this directly
A. Western blotting
B. Nortern blotting
C. in situ hybridization
D. in vitro translation
Electroporation
In the following statements, eukaryotic enhancers are not characterized by()
A. Its activity has nothing to do with direction (the effect remains unchanged after the sequence is reversed
B. Its function depends on the distance from the transcription initiation site
C. It can be 1 to 2 thousand bases away from the promoter
D. It can be located at the 5 'or 3' end of the gene
Which of the following description is correct about the poly(A) tail of mRNA in eukaryotes:
A. It is added after mRNA is transported into cytoplasm.
B. It is added by protein CPSF and CstF.
C. It can be added on the 3’-OH of primary transcription product.
D. It is transcripted from the poly(T) of template DNA.
1.Which is the following description about the carboxyl-terminal domain (CTD) in RNA polymerase II is NOT correct:
A. It binds to the mediator complex.
B. Its phosphorylation is related to the kinase activity of TFIIB.
C. The lack of CTD domain of RNA pol II would make the transcription not able to initiate.
D. The phosphorylation of its S5 site makes the change from initial polymerase form to elongation polymerase form.
E. The phosphorylation of its specific site will recruit many factors for RNA splicing and transcriptional termination.