What is the purpose of adding isopropanol:____
A. complete grinding of the sample
B. polysaccharide precipitation
C. protein denaturation
DNA precipitation and elimination of small RNA molecules.
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Which of the following statement about nucleic acid hybridization is correct:____
A. It could happen between two DNA strands from different sources
B. It could happen between DNA and RNA strands from different sources
C. It could happen between RNA strand and its encoded peptide
D. Hybridization process is based on the DNA denaturation and renaturation
E. The hybridization techniques could be used to study the structure and function of nucleic acids.
Which of the following statement about DNA renaturation is correct:____
A. The renaturation takes place among two complementary strands of the denatured DNA molecule
B. The bigger of the DNA molecule is, the longer of the renaturation time will be
C. The heat-denatured DNA would renature only if it is cooled down slowly
D. The denaturation could happen among DNA and RNA strands
E. The heat-denatured DNA would quickly renature under the low temperature
Which of the following reactions are included in renaturation process:____
A. The formation of the hydrogen bond
B. The formation of the phosphodiester bond
C. The formation of the phosphoester bond
D. The formation of the base stacking force
E. The formation of the covalent bond
Under the melting temperature, what happened to the DNA double helix:____
A. The double helix has been untied completely
B. The double helix has been untied about 50%
C. The UV absorption at 260nm increased
D. Part of hydrogen bonds between bases have been broken
E. All of the G-C base pairs disappeared
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