Which of the following statement about DNA renaturation is correct:____
A. The renaturation takes place among two complementary strands of the denatured DNA molecule
B. The bigger of the DNA molecule is, the longer of the renaturation time will be
C. The heat-denatured DNA would renature only if it is cooled down slowly
D. The denaturation could happen among DNA and RNA strands
E. The heat-denatured DNA would quickly renature under the low temperature
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Which of the following reactions are included in renaturation process:____
A. The formation of the hydrogen bond
B. The formation of the phosphodiester bond
C. The formation of the phosphoester bond
D. The formation of the base stacking force
E. The formation of the covalent bond
Under the melting temperature, what happened to the DNA double helix:____
A. The double helix has been untied completely
B. The double helix has been untied about 50%
C. The UV absorption at 260nm increased
D. Part of hydrogen bonds between bases have been broken
E. All of the G-C base pairs disappeared
Which of the following statements are correct about Tm value of DNA:____
A. It is directly related to the bases sequence order of DNA
B. It is related to the length of DNA
C. It is directly proportional to the percentage of G-C base pairs
D. The higher G+C/A+T value is, the higher Tm value is
E. Tm value represents the concentration of DNA after denaturation
DNA denaturation refer to:____
A. Depolymeriztion of polynucleotide chains
B. DNA molecule turns to double helix from supercoil
C. The phosphodiester bond in the DNA molecule has been broken
D. The hydrogen bond between bases has been broken
E. The complete hydrolysis of the nucleic acids
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