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Single-strand binding protein in the replication fork can separate two strands of DNA and prevent the complementary pairing of bases.()

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In the process of DNA replication, the leading strand is synthesized in the direction of 5’→3’, while the lagging strand in the direction of 3’→5’.()

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When DNA topoisomerase II in yeast is mutated, DNA replication process would not be affected, but its chromosome cannot be separated in the process of mitoses.()

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Which of the following is correct about the Okazaki fragment:()

A. It is synthesized by DNA polymerase
B. It is the discontinuous fragment in the lagging strand.
C. It is synthesized in the direction of 3’→5’.
D. It has free 3’-OH end.

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Which of the following statement is correct about DNA replication:()

A. conservative
B. in the direction of 3’→5’
C. 4 kinds of dNMP are participated.
D. need DNA ligase.
E. need RNA primer
F. need DNA polymerases

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