When DNA topoisomerase II in yeast is mutated, DNA replication process would not be affected, but its chromosome cannot be separated in the process of mitoses.()
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Which of the following is correct about the Okazaki fragment:()
A. It is synthesized by DNA polymerase
B. It is the discontinuous fragment in the lagging strand.
C. It is synthesized in the direction of 3’→5’.
D. It has free 3’-OH end.
Which of the following statement is correct about DNA replication:()
A. conservative
B. in the direction of 3’→5’
C. 4 kinds of dNMP are participated.
D. need DNA ligase.
E. need RNA primer
F. need DNA polymerases
Which of the following statements is correct about the synthesis of DNA lagging strands in the replication:()
A. The synthesis of lagging strand is semi-conservative.
B. The synthesis of lagging strand is discontinuous.
C. The synthesis of lagging strand is asymmetric.
D. Lagging strand is synthesized in the form of Okazaki fragment.
E. Several DNA polymerases are participated in the synthesis of lagging strand.
Which of the following statement is correct about DNA topoisomerase inE.coli:()
A. to catalyze the ligation of gaps in DNA double strands.
B. to release the supercoiled structure of DNA ahead of replication fork.
C. to break hydrogen bond and base stacking force and improve the extension activity of DNA polymerase.
D. to break phosphodiester bond of DNA and separate two strands of DNA.
E. to hydrolyze ATP and connect two strands of DNA.
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