To clone the green fluorescent protein gene downstream of the promoter of proP,what is the purpose of this?()
A. It causes the mutation of proP gene
B. Inducing the transcription of proP gene
Construction of gene library containing green fluorescent protein
D. Green fluorescent protein as a reporter gene to report the promoter activity of proP gene
E. The first step of cloning proP gene into other host cells
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What is the function of aminoacyl tRNA synthetase?()
A. Perform transpeptidyl reaction
B. Help tRNA find the A site of ribosome
C. Binding to ribosome binding site sequence of tRNA
D. Transcription of tRNA gene
E. Attaching amino acids to specific tRNA
When using bacteria to express mammalian proteins, cDNA is more commonly used than genomic DNA.Which of the following is the most appropriate explanation?()
A. Similar sized cDNA is easier to clone than genomic DNA
B. It is easier to clone RNA than DNA.
C. It's impossible to clone the whole gene coding region
D. Most eukaryotic genes contain introns, which cannot be removed in bacteria
E. Most eukaryotic promoters don't work in bacteria
What is not used in PCR?()
A. Taq polymerase
B. Restriction endonuclease
C. Oligonucleotide primer
Deoxynucleoside triphosphate
Gene chip is formed by fixing a large number of DNA probes with known sequences on the surface of a microscope carrier. Is this tool most useful for the following experiments?()
A. Predicting the presence of specific metabolites in cells
B. Comparison of newly synthesized nuclear RNA and cytoplasmic RNA to locate introns
Compare RNA produced under two different physiological conditions to understand gene expression profile
D. Compare the proteins expressed under two different physiological conditions to understand their functions
Evaluate gene linkage
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