题目内容

When using bacteria to express mammalian proteins, cDNA is more commonly used than genomic DNA.Which of the following is the most appropriate explanation?()

A. Similar sized cDNA is easier to clone than genomic DNA
B. It is easier to clone RNA than DNA.
C. It's impossible to clone the whole gene coding region
D. Most eukaryotic genes contain introns, which cannot be removed in bacteria
E. Most eukaryotic promoters don't work in bacteria

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What is not used in PCR?()

A. Taq polymerase
B. Restriction endonuclease
C. Oligonucleotide primer
Deoxynucleoside triphosphate

Gene chip is formed by fixing a large number of DNA probes with known sequences on the surface of a microscope carrier. Is this tool most useful for the following experiments?()

A. Predicting the presence of specific metabolites in cells
B. Comparison of newly synthesized nuclear RNA and cytoplasmic RNA to locate introns
Compare RNA produced under two different physiological conditions to understand gene expression profile
D. Compare the proteins expressed under two different physiological conditions to understand their functions
Evaluate gene linkage

Nucleic acid was first found by ()

A. Boysen Jensen
B. Friederich Meischer
C. Johansen
D. Went

Here is a mRNA of prokaryote. 5'-GGCGAAC()GAGGCTAGAUGCACCCUUCGCAGUACGU-3'. The order of the codon to be translated is().

A. -AUG-ACG-CUU-CCC-ACG-UAG-CTA-
B. -AUG-GAG-GCT-AGA-UGC-ACC-CUU-CGC-AGU-ACG-
C. -AUG-UUC-GCC-
D. -CTA-GAU-GCA-CCC-UUC-GCA-GUA-CGU-
E. -AUG-CAC-CCU-UCG-CAG-UAC-

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