题目内容

The role of RNA primer during DNA replication is:()

A. to guide DNA polymerase to bind with DNA template
B. to provide 5’ phosphate end
C. to provide attachment sites for four kinds of NTP
D. inducing synthesis of RNA
E. to provide 3’-OH end to initiate synthesis of new DNA

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Which of the following fragment is complementary with TAGCAT during DNA replication:()

A. TAGCAT
B. ATGCTA
C. ATCGTA
D. AUCGUA
E. AUGCUA

Which substrate in the following can be catalyzed by DNA polymerase to form DNA molecule:()

A. dNTP
B. dNDP
C. dNMP
D. NTP
E. NDP

A double-stranded DNA fragment that is isotopically labeled with 15N, was cultured for n generations in the medium containing 14N. So the number of double-stranded DNA containing pure 14N is:()

A. 2^n+1
B. 2^n-1
C. 2^n-2
D. 2^n
E. 2^n+2

The technique that confirms semiconservative replication of DNA is:()

A. Sanger method
B. density gradient centrifugation
C. α-complementation
Dot blot
E. Western blot