The technique that confirms semiconservative replication of DNA is:()
A. Sanger method
B. density gradient centrifugation
C. α-complementation
Dot blot
E. Western blot
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Which of the following statements about the chromatin remodeling complex are correct:____
A. The chromatin remodeling complex consists of SWI/SNF, ISWI and SWR1 family.
B. The chromatin remodeling complex contains domains for recognition of modification site of histone.
C. SWR1 family can replace the H2A-H2B dimer of the nucleosome to H2A.Z-H2B dimer.
D. ISWI family can make DNA molecule slide on the core histone octamer, changing the arrangement of nucleosome and shortening the distance of neighboring DNA.
Which of the following statement about the chromatin remodeling is NOT true:____
A. It is about the change of the structure and position of chromatin.
B. The location of nucleosome in DNA strands keeps unchanged.
C. It is closely related to the modifications in the N terminal of histone.
D. The complex for chromatin remodeling possess ATPase activity and can break the interaction of DNA and histone.
Which of the statement about the modification of histone is correct:____
A. The acetylation of Lyc residue in the N terminal of histone can strengthen the interaction between different histones.
B. Chromo domain can recognize the acetylation site of histone.
C. The acetylation and phosphorylation of histone reduce the affinity between histone and DNA.
D. Bromo domain can recognize the methylation site of histone.
Which of the following statement about the location of nucleosome is NOT true:____
A. The interactions between nucleosome and DNA are dynamic.
B. The interactions of nucleosome and DNA are not sequence specific.
C. A nucleosome consists of DNA molecule with length of at least 150bp.
D. The DNA sequences don’t affect the location of nucleosome at all.
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