Which of the following statement about the initiation of translation in eukaryotes is NOT correct:()
A. During the translation initiation process of eukaryotes, some initial factors binds to the small subunit of ribosome and some factors binds to 5 ' end cap of mRNA.
B. Translation initiation factors may be modified by a variety of intracellular kinases and phosphatases, which would affect the efficiency of translation initiation.
C. During the translation initiation process of eukaryotes, the correct pairing of initiator tRNA and the start codon will lead to the release of various initiation factors.
D. Phosphorylation of eIF2 in eukaryotic cells would strengthen the interaction of eIF2 and eIF2B, contributing to improve the efficiency of translation initiation.
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Which of the following statement about microRNA is NOT correct:()
A. Most miRNA will be separately located in the genome, only a few appear in cluster.
B. Each miRNA may target several genes, but the same gene is regulated only by one miRNA.
C. The sequence of miRNA is highly conservative in different species.
D. The regulation of translation process by miRNA relys on RNA-induced silencing complex (RISC).
In miRNA synthesis,which is the right order of the following molecules to produce or to work:①Dicer protein②RISC complex③primary miRNA④pre-miRNA⑤Drosha protein()
A. ④⑤①③②
B. ③⑤②④①
C. ②①③⑤②
D. ③⑤④①②
when DNA chip is used for analysis of differential gene expression, the purified mRNA can be directly used to hybridize with DNA chip and we just need to prevent RNA sample from degradation of ribonuclease.()
in the real-time PCR, Both fluorescein-embedded method and probe method can be used for quantitative detection based on that the fluorescence signal intensity is positively relative to the amount of PCR product.()
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