At the isoelectric point, the net charge of the protein molecule is 0.
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Which of the following are acid amino acids?
A. Glutamate
B. Lysine
C. Arginine
D. Aspartate
E. Alanine
Primary structure of protein
A. Is the basis of spatial structure
B. refers to amino acid sequence
C. It does not include disulfide bond
D. It mainly depends on peptide bond to maintain stability
E. α-helix and β-sheet are the major conformations of it
Protein denaturation is because
A. Hydrogen bond is destroyed
B. Peptide bond cleavage
C. Subunit depolymerization
D. The hydration membrane is destroyed and the charge is neutralized
E. Disulfide bond cleavage
The properties of denatured proteins are
A. Solubility drops significantly
B. Loss of biological activity
C. Easy to be hydrolyzed by protease
D. Solidification
E. precipitation