限制性片段长度多态性(RFLP)分析只能用于分析下面哪种情况的遗传病:( )
A. mRNA探针与抗体结合使用
B. 致病突变位于或与改变的限制性位点密切相关
C. 突变基因和正常基因编码的蛋白质在凝胶电泳中的迁移有差异
D. 突变不在限制性位点,所以仍然会发生切割
E. 限制性酶切片段大小不变,但其电荷变化
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Most of today’s somatic engineering uses viral vectors to transfer a transgene into target cells. Unfortunately, the vectors insert transgenes at random sites within the receiving chromosome. The outcome is necessarily somewhat unpredictable because genes are affected by their immediate environment, which in this case is not well defined. Also, a transgene might cause serious side effects if it were placed in the middle of an important existing gene. A human artificial chromosome (short HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That is, instead of 46 chromosomes, the cell could have 47 with the 47th being very small, roughly 6-10 megabase in size, and able to carry new genes introduced by human researchers.1. If we transfer an insulin gene into patient’s pancreas for gene therapy, we call the insulin gene a ______ .2. The therapy may not be predictable because (a) ______ 3. and (b)______ 4. HACs can be used by______ .5. One megabase is equal to ______ kelobases.
什么是限制性内切核酸酶的星活性:( )
A. 在非常规条件下,识别和切割序列也不发生变化的活性。
B. 活性大大提高
C. 切割速度大大加快
D. 识别序列与原来的完全不同
考斯质粒(cosmid)是一种( )
A. 容量最大一种载体
B. 由λ -DNA 的cos区与一质粒重组而成的载体
C. 是一种单链DNA环状载体
D. 不能在受体细胞内复制,但可以表达
下列DNA片段中含有回文结构的是( )
A. GAAACTGCTTTGAC
B. GAAACTGGAAACTG
C. GAAACTGGTCAAAG
D. GAAACTGCAGTTTC