Topoisomerase I need to break the phosphodiester bond in sugar-phosphate backbone of DNA to unwind double helix, which would consume ATP.()
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The DNA replication in E.coli, yeast, virus are all initiated at a specific origin site, which consists of several short tandem repeats and they are the targets of initiator proteins.()
Both DNA polymerase and RNA polymerase are needed in DNA replication process.()
The deficiency of 3’→5’ exonuclease activity of DNA polymerase in E. coli would slow down the synthesis of DNA, while the fidelity of DNA would not be affected.()
Both two strands in the telomere can be synthetized by its telomerase using its RNA as template.()