Which of the following statement about DNA replication of E.coli is NOT true:()
A. Four kinds of dNMP are needed.
B. It requires DNA ligase.
C. RNA primer will be formed in the process.
DNA polymerase I is needed.
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DNA denaturation means:____
A. Depolymeriztion of polynucleotide chain
B. Supercoiled DNA molecule turn to double helix structure
C. The phosphodiester bond of the molecule has been broken
D. The hydrogen bond between bases has been broken
E. The complete hydrolysis of the nucleic acid molecule
Which of the following statement about nucleic acid hybridization is correct :____
A. It could happen between two DNA strands from different sources
B. It could happen between DNA and RNA strands from different sources
C. It could happen between RNA strands and its encoded peptides
D. Hybridization process is based on the DNA denaturation and renaturation
E. The hybridization techniques could be used to study the structure and function of nucleic acids
The Tm value of DNA decreased with the increasing of (A+T) /(G+C) value.
For purified DNA sample, the value of OD260/OD280 lower than 1.8 indicating the sample contains RNA.( )
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